Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial
Supelco Inc HEPES, 7365-45-9, MFCD00006158, 100 G
Empirical Formula: (Hill Notation) C8H18N2O4S, Molecular Weight: 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), assay : ≥99.5% (titration). HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Alkali Scientific CellPro™ PBS 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Medchemexpress LLC Hepes, NaCl, sucrose, pH 7.2 buffer | 50 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is used to resuspend human erythrocyte ghost preparations. The buffer serves as the final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts.
- Used as a biochemical assay reagent
- Aids in the resuspension of human erythrocyte ghost preparations
- Appearance is liquid
- Color is colorless to light yellow
- Storage in pure form: -20°C for 3 years, 4°C for 2 years
- Storage in solvent: -80°C for 6 months, -20°C for 1 month
United States Biological Corporation TRIS HCL BUFFER 1M PH7.5,500ML
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
Alkali Scientific QuickSilver TBS-T Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 500g
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | Purity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 500g
Bioworld STEBuffer (1X), 1 L
STE Reagent Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, ready to use 1X solution Technical Information:NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Bioworld TAE Buffer 50X, Liquid Concentrate 1L
TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended. Appearance Form : Liquid, Appearance Color : Clear. 1L
Bioworld STE Buffer (10X), 1 L
STE ReagentCommon reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, 10X solutionTechnical Information:1X concentration solution containsNaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Bioworld TAE Buffer, Quick-Mix Pack (25X), 4 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Research Products International Corp PIPES SODIUM SALT, 100 GRAM
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5